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Second, in an effort to detect the net effect of associations from multiple variants, we tested for global haplotypic association.
Using increasing amounts (20; 100; 200; 1,000 ng) of the different proteins, GST alone, GST-Sg4E-BP (WT: Wild Type), YALA, 4xA and 4xE variants, we tested their capacity to inhibit the translation of a capped Renilla Luciferase (R Luc) mRNA in the sea urchin cell-free translation system prepared from fertilized eggs.
Given the strong evidence for sterol regulation of HMGCR splice variants, we tested whether there are also sterol-regulated changes in the relative amounts of alternatively spliced to full-length transcripts of other key regulatory genes involved in cholesterol metabolism - LDLR, HMGCS1, MVK, and PCSK9.
GA- SUCNR1 relationships emerged for two of the three common DNA sequence variants we tested (rs13315275, Pexact=0.005; rs9811297, Pexact=0.031).
Using these criteria to select variants we tested for ASE in 56,825 SNPs when only interrogating the Geuvadis samples and tested for ASE in 225,562 SNPs when using all 1,262 samples.
Hypothesizing an optimal intermediate number of MHC class IIB sequences variants, we tested the effect of MHC on these residuals by fitting a quadratic polynomial as the simplest function possessing a minimum.
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Thus, the cassette containing both the Xenopus 5′-UTR translational enhancer sequence and the intron-containing rabbit β-globin polyadenylation sequence provided the highest FP expression among the design variants that we tested.
As previous reports suggested that individual cells could express distinct splice variants 11, we tested whether individual MEC1AID-SRC cells could be discerned, which fail to produce AID-FL but express alternative splice variants that are not recognized by specific antibodies, which are variants AID-ivs3 and AID-ΔE4 (Supporting Information Fig. 2).
Moreover, to demonstrate the utility of these new allelic variants, we have tested them for PVY resistance.
To pursue unresolved issues from the previous WGS study that included unconfirmed variant calls and the challenging interpretation of incidental variants, we further tested the subject's DNA with the latest version of ES methods.
To demonstrate that true functional genomic FRT-like sequences can also serve as substrates for the respective Flp variants in mammalian cells, we tested Flp variants that can recombine FRT-like sequences located in the human b-globin locus (FL-61204, FL-63904, and FL-71362, Figure 1B) in CHO cells (Figure 2).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com