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For GFP promoter variants, we measured fluorescence activity as the change in GFP molecules per cell per unit time (Figure S5a).
After 24 h exposure to the sTRAIL variants we measured apoptosis and found that HeLa (Fig. 2a) and Colo205 (Fig. 2b) cells showed higher cell death levels with sTRAILDR4 and sTRAILDR5, respectively.
To determine the extent to which classic Notch phenotypes are associated with RALX outcrossed lines carrying rare opa variants, we measured the frequency of duplicated macrochaetes on the notum of adult flies in both parent and the RALX introgressed lines carrying variant Notch opa alleles.
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[35] To investigate how changing the pH of the enzyme assay affects the activity of the thialysine-165 variant, we measured the pH-activity profile of both wild-type and thialysine-containing S. aureus NAL.
In an effort to increase sensitivity and throughput relative to existing kinetic assays, and to enable robust comparison between variants, we simultaneously measured the interaction between GCK and each variant GKRP (including the common p.P446L variant) using a sensitive, quantitative and miniaturized homogenous time-resolved fluorescence (HTRF) assay (Fig. 2A).
To test whether the nonsynonymous variants affected iC3b binding, we measured the ability of transiently transfected COS-7 cells to associate with iC3b-opsinized sheep red blood cells (sRBCiC3b) by using an Association Index.
To determine the variant quality score threshold, we measured genotype concordance between individual-matched WGS from lymphoblast and fibroblast samples from seven individuals, which are expected to have minimal discrepancies.
To assess the dose-dependence of MyoD and its engineered variants on myogenic gene induction, we measured marker expression at various doses of MyoD by manipulating the concentration of doxycycline.
In this study we measured two variants of AGD: The first was measured from the cephalad insertion of the penis to the center of the anus (AGDAP; Figure 1, point 1 to point 3), and the second was measured from the posterior base (first fold) of the scrotum to the center of the anus (AGDAS; Figure 1, point 2 to point 3).
To test if release of TGFα was linked to MMP-1 expression in the brain metastasis-derived variants of MDA-MB-231, we measured both MMP-1 and TGFα concentrations in culture supernatants of MMP-1 shRNA expressing cells and control cells by ELISA.
We measured several psd module variants at very low amounts of blue light (5 μmol m−2 s−1) and observed qualitatively similar results.
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