Sentence examples for variants was then from inspiring English sources
The clinical significance of the variants was then analyzed with MitoMaster (http:// www.mitomap.org/MITOMASTER/WebHome).
Search for variants was then made with the unified genotyper module of GATK.
The effect of applying these filters on colocalization with the different types of BRCA1 sequence variants was then determined.
This high-confidence set of variants was then used for base-quality recalibration using GATK, with the default set of covariates.
Each of the remaining 996 variants was then annotated with information available from HGMD, ClinVar and LSDBs and for the predicted consequence (e.g., frameshift, splicing and termination).
The transcriptional activity of TBX1 and the TBX1 variants was then measured by comparison of the firefly luciferase reading with the Renilla luciferase control (dual luciferase assay).
Variants are then tested empirically and further variants are created based upon their performance.
These variants were then generated by site-directed mutagenesis in the MCPH1 construct and expressed in yeast (Figure 4c).
The resulting GALR3 agonist or antagonist stabilizing variants were then further used for recombinant protein expression in transfected insect cells.
A few of these variants were then purified in order to analyse their RNA binding properties by surface plasmon resonance and to check their structural integrity by NMR.
The family of hierarchical routing techniques and several of its variants are then overviewed, in addition to other techniques such as move-based heuristics and iterative deletion.
