Sentence examples for variants was performed from inspiring English sources

Identification of microcystin variants was performed using an Agilent 1100 Series LC/MSD Trap XCT Plus high-performance liquid chromatograph mass spectrometer (Agilent Technologies).

CFD analysis of the classic Cabrol procedure and a number of its variants was performed.

Functional annotation of variants was performed using Polyphen-2 [22] and the Combined Annotation Dependent Depletion method (CADD) [23].

Protein structural analysis with these amino acid variants was performed by using I-Mutant, FOLD-X, HOPE, NetSurfP, Swiss PDB viewer, Chimera and NOMAD-Ref servers to check their solvent accessibility, molecular dynamics and energy minimization calculations.

Detection of variants was performed with the GS Reference Mapper Version 2.0.0.12 (Roche).

Screening of cDNAs for different splice variants was performed with intron spanning exonic primers listed in Table 1.

Absolute quantification of splice variants was performed using a serially diluted standard containing the same sequence as the target amplicon.

Numbering of sequence variants was performed following the HGVS Mutation Nomenclature Recommendations (http://www.hgvs.org/mutnomen/recs.html), using reference sequence AF301906.1.

Agroexpression of ipiO variants was performed using vector pGR106 [46] in Agrobacterium tumefaciens strain GV3101 [47] as previously described [48].

Surface plasmon resonance, to measure the binding between PA and receptor variants, was performed using the Biacore 2000 system.

Relative quantification of MAb and its variants was performed by manual integration of the chromatographic peaks and comparison of the respective areas.