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The hemolytic activity of cathelicidin (bactenecin) variants was much less than that of indolicidin due to their lower hydrophobicity.
The helix destabilizing activity of Q28A, G38D, R39A, Q28A/G38D and Q28A/G38D/R39A was found to be much more compromised as Tm of DNA with these variants was much higher (Table 4).
We also noted that the concordance (1.1 %) of novel variants was much lower than that of known variants (71.2 %).
For imputed and real sequence data, the number of segregating variants was much smaller for the Jersey population than for the Holstein-Friesian population.
Only one mutation in exon 1, a transition G128A that alters aa 43 R to K, was found and predicted to be tolerated, so the number of natural variants was much higher than that obtained with induced variation.
In East Asia and Oceania the average nucleotide diversity of the loss-of-function variants was much lower than that of the variants that do not affect OCT1 function (ratio of 0.03) and was significantly lower than the theoretically expected heterozygosity (Tajima's D = −1.64, P < 0.01).
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Most are of serous or mucinous type with other morphological variants being much more uncommon.
Both variants were much less potent inhibitors of fungal growth than RGFRRR.
It is also noteworthy that miR-142 is the most highly expressed miRNA in naïve T cells, the concentration of each of the individual miR-142 variants is much higher than most miRNAs expressed in naïve T cells.
Compared with Caucasians, the MAFs of these two variants were much rarer in the Chinese populations (0.05 vs. 0.40 for rs10010131 and 0.125 vs. 0.45 for rs8050136) [21], [21], which reduced the statistical power of our samples dramatically.
We observe that the GCI area under the curve with currently known variants is much less than that of the optimal theoretical genetic models, which suggests that many additional unknown genetic variants and/or interactions are expected to affect these diseases.
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