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The identity of all splicing variants was confirmed by sequencing.
The differential expression of these genes in FF and ff variants was confirmed by qRT-PCR.
Transmission of multiple viral variants was confirmed in 8 of 25 (32%; 95% CI 14% to 50%) cases.
Segregation of both variants was confirmed by Sanger sequencing.
In all cases, the identity of the amplified variants was confirmed by sequencing.
Cosegregation of the variants was confirmed by Sanger sequencing analysis of the variants in family members.
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These variants were confirmed by Sanger sequencing.
The use of CXCR4 coreceptor by minor variants is confirmed with sufficient suppression of RLU by a CXCR4 inhibitor.
The MME variants were confirmed by Sanger sequencing of the entire MME coding region in all CH patients; no additional rare variant was detected except for a synonymous SNP (rs200455903).
Putative variants were confirmed by traditional Sanger sequencing of fresh LR-PCR amplicons.
All sequence variants were confirmed by sequencing the products of independent PCR reactions in both directions.
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