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This study demonstrates that endonuclease mutants with altered specificity and non-lethal activity can be evolved towards more active variants using a laboratory evolution strategy.
Here, we describe a real time quantitative PCR (qPCR) approach to investigate relative changes in the expression of STREX and ZERO splice variants using a newly designed set of probes and primers for TaqMan-based qPCR analysis of cDNA from the rat dentate gyrus at different time points following pilocarpine-induced status epilepticus.
The International Civil Aviation Organization (ICAO) classifies variants using a shortened code formed by combining the model number and the variant designator (e.g. "B741" for all −100 models).
Next, we analyzed the ssDNA-binding properties of the terminal deletion variants using a GMSA approach.
We recalculated the number AS variants using a new method, which gave these 'classes' a smaller weight.
In each design, we related the 5 different sum-scores to the genetic variants using a one-way ANOVA with three groups (i.e., the genotype groups aa, Aa, and AA), yielding 5 different p-values.
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Therefore, to experimentally identify residues in this region of DmsD that permitted signal peptide binding, we created 2 random libraries of DmsD variants using an NNK library approach that targeted residues W72/L75/F76 in the putative binding pocket [43].
If leather isn't your thing, other variants use a hammered pattern back finished in metal or ceramic-painted white plastic.
All of the variants use a foot-type parking brake, welcome the driver whenever the ignition is set to "ON", displaying on the central console "Welcome to Camry".
This study describes a simple and specific molecular detection test for the PVYN-W variant using a PCR protocol based on the recombinant point within the HC-Pro/P3 region of PVYN variants (PVYNTN, PVYN-W).
In 2013, they reported a catalytic enantioselective variant using a bifunctional cinchona alkaloid-derived thiourea catalyst and stoichiometric titanium complex.
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