A total of 3,620,895 single nucleotide substitutions and 509,994 indels were identified in this sample, and we further filtered the variants to only keep the ones with a SNP quality score > = 30.
The remaining variants were filtered to keep only biallelic variants for which the two parents, BT×623 and IS3620C, were each homozygous for different alleles and to keep only variants that were genotyped with a GQ score ≥ 20 in ≥ 25% of the samples.
Resulting nuclear variants were hard filtered to keep variants with a Phred scaled quality score of 20 or higher (a measure of quality of DNA sequence) [ 21, 22].
The variants were filtered to keep the ones where the depth of coverage was > = 5 and < = 60 for all chromosomes except the mitochondria.
A confidence filter was applied to keep variants with call quality >20 for the samples and, excluded variants in the top 5%% most exonically variable 100 base windows in healthy public genomes and the top 1 % most exonically variable genes in 1000 genomes.
Megayacht owners, aided by a fleet of lawyers, management companies and banks, are developing increasingly complex variants of these ploys to keep a step ahead of the tax man even as the authorities strive to close down what they see as scams.
To keep the model variants as simple as possible Eqn (4 6) take into account only homotypic pairing of FPs.
As functional information is constantly evolving, end users are forced to keep data sources and variant annotation up to date with every release of new information.
Furthermore, we decided to keep Topaz and ECFP, Aequorea victoria GFP variants, which have nearly identical DNA sequences, separate by recombining them into the two different BAC subclones.
In order to keep the difference between the two categories of DNA variants, we propose a clear distinction between a SNP and a somatic mutation.
