This is the MCF10 progression panel, which includes the normal immortalised MCF10a cells, Ha-Ras transformed MCF10neoT cells, premalignant MCF10AT1 cells, a cloned xenograft lesion of MCF10AT1 cells (MCF10DCIS.com), and malignant variants that form invasive tumours with varying degrees of differentiation in xenografts (MCF10CA1, h, d, and a) [ 34, 35].
While the domain pattern in a charge-free BiFeO3 film consists of only two polarisation variants, we observe complex patterns with four coexisting variants that form within the charged layers at sufficiently high induced fields.
These were readily identified along with black variants that formed more highly melanized colonies.
To underscore the issues related to background variation in the commonly used zebrafish lines, we detected 669 variants that formed premature stop codons in at least one transcript, 105 of which were homozygous mutant in both sexes (Table 2).
Notably, stalling was observed for a variant that formed a stable 10-stranded intermediate but not for one in which this intermediate was unstable.
BSE, vCJD, and several other neurodegenerative diseases are believed to be caused by so-called prion proteins, which can occur in two forms: the normal variety, called PrPc, and a misfolded variant that forms clumps in the brain (PrPSc).
This is strongly supported by the gene expression of prostanoid receptors, FP, and its splicing variants, notably the altFP4 variant that forms a bimatoprost-sensitive activation site with FP (FP/altFP4; refs. 20, 30), in scalp hair follicles and isolated dermal papillae and CTS in vivo.
The microstructure of different crystallographic phases and variants that is formed for a given load is then captured by an estimate of the quasiconvexification of the resulting energy landscape.
On the contrary, in smaller austenite grain, Bain grouping of variants that always forms low angle grain boundary and favors crack propagation dominates the transformation, and it will promote the crack to propagate through the entire Bain zone and then yield large long crack.
This was employed to identify the particular variants that formed in each grain.
Specific variants occur at specific positions, generally with only two of the possible four bases observed at these positions, for example if the wildtype is C then only G mutants will be observed but not A or T. It is therefore possible to consider building a library that contains all possible sequence variants that can be formed by combinations of previously observed variants.
