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In this study we characterized the cross-reactivity of several high-affinity 2C TCR variants that contained mutations only in the CDR3alpha loop.
DNA constructs for the expression of deletion variants that contained N-terminal parts of the proteins were prepared by inserting a stop codon after residue 550 for CMTr1 and after residue 430 for CMTr2.
Mass spectrometry strongly suggested that Microcystis strains NPLJ-4 and RST 9501 produced new microcystin variants that contained Met instead of D-Leu (Table 1, Figure 1b).
DOI: http://dx.doi.org/10.7554/eLife.03069.007 Using dimeric rescue constructs did not allow us to test Stu2p variants that contained only a single functional TOG domain of either kind.
Kinase activity assays were performed in duplicate in gel filtration buffer (BME was excluded from the buffer for chemically modified variants that contained dehydroalanine) using a LabChip EZ Reader II system (Caliper Life Sciences) with 1.5 μM fluorescein-labeled Caliper Peptide 21 (Flu-LRRASLG) as the substrate and 12-point 2-fold serial dilutions of ATP.
We screened our population samples for Hg HV1 variants that contained the same HVS-I mutation 16355 observed in Georgian Jews.
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Novel inhibitors are sought that increase potency against variants that contain the Tyr181Cys mutation.
Here, we constructed a series of variants that contain a single tryptophan at different locations along the polypeptide chain.
This study compared the activity of three histone-derived antimicrobial peptides buforin II, DesHDAP1, and parasin with variants that contain only lysine or arginine cationic residues.
Re-introduction of L857 back to the S gene of Vero-adapted IBV allowed recovery of variants that contain the introduced L857.
A characteristic of these repetitive sequences is their ability to give rise to variants that contain increased or decreased numbers of repeats.
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