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On the other hand, W99V and W99A variants show a remarked increase and decrease, respectively, of the maximal signal amplitude, compared to WT, while not deviating significantly from WT in EC50 concentrations (Figure 2B).
It is noteworthy that in our experimental model, K562 and Lama84 cell lines, both derived from patients in blast crisis, the resistant variants show a marked increase in Bcr/Abl expression levels, obviously implicated in IM resistance.
The bZIP containing PtAu1a variants show a low kinetic stability and mostly elute as monomers from the gel- filtration column.
All three deimmunised variants show a reduction in T cell proliferation in comparison with the wild-type ABD001.
All variants show a transcriptional activity similar to wild-type tTA in the absence of dox, but they respond differentially to the presence of dox.
Many species with sexual and asexual variants show a pattern of geographic parthenogenesis where asexuals have broader and higher-latitude distribution than sexuals.
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In library H236 three variants showed a higher stability: H236K, H236I and H236R.
Variants showing a residual activity greater than the wildtype enzyme plus standard deviation were considered as hits.
CYP102A1 variants showed a preference for hydroxylation at the ω-1 position of lauric acid, and myristic acid, and at the ω-2 position for palmitic acid.
However, R39A, Q28A/G38D, Q28A/R39A, Q28L/R39A, R39A/G38D and Q28A/G38D/R39A variants showed a remarkable decrease in their thermal stabilities (Table 3).
Our analysis of nuclear encoded variants shows a trend suggesting the possible involvement of the NDUFS2 gene from Complex I.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com