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Sanger sequencing was used to assess small variants of interest in our dataset [28].
The study design depends on the effect size of the variants of interest.
All the mentioned approaches can be applied to migraine according to the effect size of the variants of interest.
No single BPS tool offers sufficient capabilities and the flexibility to resolve all the possible design variants of interest.
Among the genetic variants of interest are G894T endothelial nitric oxide synthase and G1958A methylenetetrahydrofolate dehydrogenase 1 gene polymorphisms.
The experts' ideas suggest that the preferred NGS approach depends on the expected effect size and the frequency of the variants of interest.
However, there are still considerable difficulties in discovering common genetic variants of interest.
Targeted assembly is a powerful method for interrogating large data sets for the presence of sequence variants of interest.
With these resources it is now possible to pick tag SNPs that are in LD with specific HLA variants of interest (i.e. have high r2 values).
Rather, NGS data sets can be mined for the presence of sequence variants of interest by localized assembly, which is a faster, easier, and more accurate approach.
Thus, TASR has particular utility for finding or confirming, through local assembly, the presence of specific sequences or sequence variants of interest.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com