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The 50 up- or down-regulated transcripts with the highest- fold change (including splice variants, not shown in table) in potato leaves following treatment with 10 mM BABA.
Two rare variants (not shown) suggest visual impairment and color vision deficiency, but there is no indication that these are issues for this woman.
This was confirmed by SDS PAGE and Western blot experiments showing similar β-tubulin levels in COS1 cells transfected with the different parkin variants (not shown), thus assuring similar amounts of cellular input material.
A Blast search of whole genome shotgun data from an ongoing Dunaliella salina genome sequencing project available in Trace Archive (http://www.ncbi.nlm.nih.gov/Traces/) revealed the presence of short stretches of telomere-like sequences and also of long tracts consisting of the Arabidopsis-type variant or of a mixture of the human-type and the Arabidopsis-type variants (not shown).
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Background phosphorylation was also present in the AVA-SS variant but could not be detected in the AVA-AA variant (not shown).
In the high-resolution narrow scan of its 9 GHz EPR spectrum, the tryptophanyl contribution observed for WT DyP completely disappeared (the spectrum of the W377S/Y147S variant, not shown, being nearly superimposable).
As had been determined previously for other proteins [14], [15], multiple tryptophan to phenylalanine replacements caused a serious reduction in stability of the variants (data not shown) and therefore variants containing multiple mutations were not analysed further in this study.
Once we filtered out variants that were not located in the regions defined by the Illumina TruSeq exome bed file, we went from hundreds of thousands of putative variants (data not shown) to, on average, about 23,000 variants (SNVs and indels) per pipeline (Table 3).
No significant differences were noted in the distribution of the three main NOD2 allele variants (data not shown), however the presence of two variant alleles trend to be higher in pediatric-onset than in adult-onset patients (p=0.12) (Table 1).
Assembly of the 5S rRNA gene confirmed three variants (data not shown); downstream of the 5S rRNA gene, all three variants differ from Methanoculleus marisnigri JR1, indicating a different genomic context for the rrn cluster.
For the A6M3 site, high amplicon intensity with no variation was detected for the orange and green aphid variants (data not shown).
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