To evaluate whether missense variants inhibit the function of the CHEK2 protein, an in vitro kinase activity test based on a CHEK2-specific substrate peptide carrying a C-terminal SOX was developed [ 42].
Tate CM, Pallini R, Ricci-Vitiani L, Dowless M, Shiyanova T, D'Alessandris GQ, et al. A BMP7 variant inhibits the tumorigenic potential of glioblastoma stem-like cells.
However, the two splice variants are mutually exclusive, and the 11a variant inhibits the expression of MenaINV.
Whereas cells transfected with SDC4A198del-GFP exhintermediatediamountsunts of PKCα expressed at the cell membrane, the SDC4L188QQ-GFP varinhibitsibithethe PKCα translocation (Fig. 5D; supplementary material Movie 4).
The protein phosphatase (PP) inhibition assay revealed that all MC structural variants inhibited PP-1 in the nanomolar range (Table 4).
Synthetic LL-37 variants inhibited commercial MAPS CPS similar to the laboratory purified meningococcal CPS polymers.
In accordance with a study showing that specific VacA variants inhibited CagA-dependent cell elongation, CagA reduced VacA-mediated apoptosis and vice versa, underscoring the interfering functions of pathogenic factors expressed by H. pylori [ 56, 57].
Variants that inhibited the E2 also prevented the enzymatic activity.
All ES variants inhibited Erk phosphorylation (Fig. 3d).
Four splice variants of the gene with antagonistic effects were identified in mouse; overexpression of Irak2a and Irak2b potentiated activation of NFĸB pathway, whereas Irak2c and Irak2d variants inhibited it [ 24].
