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We analyzed the distribution of m-spinesin variants in mouse tissues, using RT-PCR with variant-specific primer sets.
Similarly, the knockouts and overexpression of genes encoding different H1 variants in mouse produced no evidence that the variability of H1 in this complex multicellular organism is indeed functionally important.
Furthermore, there are a variety of Ano1 splicing variants in mouse heart and these splicing events are highly conserved between human and mouse (O'Driscoll et al., 2011).
Cloning identified two splice variants in mouse brain lacking either exon 3 or exon 3 together with exon 4 (Fig. 1c).
For the NFKB2 promoter, we did not find any IRF family TFBSs in all species, apart from 1 weak binding site for the IRF3 in 2 of 4 promoter variants in mouse, overlapping stronger the NF-κB family binding sites.
Given the growing recognition of the biological importance of genomic structural variants for human diseases, we anticipate that TRACER will be a useful resource to rapidly engineer allelic series of structural variants in mouse orthologous intervals, helping to create novel models of human genomic disorders.
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However, the activities of sphinganine variants in mice have generally not translated well to studies of human iNKT cell responses.
To maximize the sensitivity of detecting affects of genetic variants in mice, variables have been minimized through the use of inbred mouse lines, by eliminating infectious organisms and controlling environmental variables.
Single knockout H1 variants in mice show upregulation of other H1 variants and relatively mild phenotypes [ 9].
71 CLDN18 has two variants in mice: variant 1 is expressed in the lung, whereas variant 2 is expressed in the stomach.
In addition, this allowed us to identify new Jmjd6/JMJD6 splice variants in mice and humans and to analyse the genomic neighbourhood of the gene in vertebrates.
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