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Furthermore a PCR amplifying the pilin locus was performed on Z5463BC without starting from an isolated colony and cloned in E. coli, eight of these clones were sequenced, they identified an additional two pilin variants in addition to the above four (Fig. 7).
We created a series of double and triple variants in addition to the single variants H236K, H236I and H236R (see Table 3).
Since this strain also produces several minor MC structural variants in addition to the most dominant compounds [4], we were interested in their structural elucidation.
Two other recent papers evaluated a set of genetic variants in addition to the clinical risk factors [10], [11].
Note that given the "detection sample" sizes, all but two of the 18 new SNPs were detected in only one individual (accuracy checked with a replication of the sequencing for these rare variants, in addition to the double strand sequencing applied to all variants), the two remaining SNPs being detected in two individuals from different populations (see Table 3).
We selected 5 of these variants, in addition to Ala794Gly, for further analysis.
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Both a natural HpSVd-hop and -grapevine variant, in addition to an in vitro transcript of HpSVd-grapevine, were introduced into hops and cultivated over 15 years.
Optimistically, in natural data each case has one or more rare-variants in addition to the natural background variation (which would also be present in controls).
Five patients (5/52=9.6%) showed a novel variant in addition to a pathogenic allele in a recessive gene.
A new variant (in addition to a preexisting variant) was detected in posttreatment samples in 6/15 (40%) patients (Table 1; Supporting Fig. 2A-C).
Model 8 was a bit different in that it contained a moderate main effect for one genetic variant in addition to a stronger three-way interaction.
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