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Then there is the M50 steel and its variants, from which bearings which serve at slightly higher temperatures in aeroengines are manufactured, based on secondary-hardened martensite.
Importantly, each nucleosome contained a unique hexanucleotide barcode that specified the histone variants from which the particular nucleosome is constructed.
The most widely used and best characterized cell line for study of acquired tamoxifen resistance has been the MCF-7 variants, from which much of our current understanding of the mechanisms of hormone resistance has derived [ 13, 14].
In these cases, selection has to be carried out on individual clones (e.g. in separate fermentation experiments), thereby severely limiting the pool of variants from which improved strains can be selected.
This library contains 256 variants from which 23 were randomly selected and subjected to single-molecule determination of the reduction rates at 75 pN (a low force at which the Michaelis Menten mechanism is the major contribution) and 500 pN (a high force which reveals the contribution of the simple chemical SN2 mechanism).
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Only one product was seen on agarose gels which, on sequencing, was found to be the shorter AC133-2 splice variant from which exon 4 is spliced out (Figure 2a and 2c).
The MRCA, a hypothetical viral sequence that represents the most recent viral variant from which a subject's viral quasispecies are descended, was reconstructed in DIVEIN [23] by the joint maximum likelihood procedure [25].
As in our recent SCAM-based studies of individual TMs, we have used a human CFTR variant from which all cysteines had been removed by mutagenesis (as described in ref (31)) and which includes a mutation in NBD1 (V510A) to increase the level of protein expression in the cell membrane.
Data from three independent experiments (each in duplicate) were used to determine IC50 values of meropenem for each PBP2 variant, from which second-order acylation rate constants were derived using eq 3: 3where (Ks/ Ks)U is the rate constant of an unlabeled compound, (k2/ Ks)L is the rate constant of Bocillin-FL, and [SL] is the concentration of Bocillin-FL used in the competition experiment.
Compared to a reference fermentation on SSBM-P (ferm. #20), growth on an SSBM-P variant from which D-glucose was omitted (ferm. #22) was still good, though at a slightly reduced growth rate, and in addition resulted in a lower maximum biomass concentration.
Our results show that fluorescence of GFP insertion reporters containing different test proteins (Fig. 4A) reflects the robustness of the GFP variant scaffolding from which the reporters are derived (Fig. 3B).
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