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Red signals a "genetic mosaic" of the parents, with different sequence variants from each.
In most cases, for a particular genetic sequence on a specific chromosome, the variants from each pair should be represented equally in the woman's blood.
At least 16 plasmid clones containing ipiO variants from each isolate were sequenced in both directions using vector-specific primers.
Our findings were in agreement with a prior study by Palmer and colleagues who reported detection of additional 1 to 10 minority drug-resistant variants from each of 26 treatment-experienced subjects failing ART using single-genome sequencing [24].
The results are shown in Figure 2. The power calculation showed that a minimum of 10 biological variants from each group are required to be confident of a 1.5-fold (50% effect size) and 2 fold (100% effect size) change in abundance between CR and CNR at the p<0.05 and p<0.005 level of statistical significance respectively.
All variants from each genome were annotated independently using Annovar Tool.
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The particular variation in the gene is common in the general population, with about a third of people inheriting both copies of the deficient gene variant from each of their parents.
We selected one variant from each representative protein domain.
Typically, GWA studies report the single variant from each gene region showing the strongest association with the trait of interest.
This is possible because a full-length cDNA represents a single splice variant from each transcription unit.
Sequencing of the acrB mutants identified only one variant from each library and no mutation was found in the lac operator for both variants.
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