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We report a murine IgG1 mAb, JAR 41, which broadly cross-reacted with fHbp sequence variants from all variant groups.
Each insect gut community contained CODH variants from all three of these clades.
A similar observation was made in HIV-1 variants from all recipients in which the number of forward mutations was the highest in Env and Nef.
HIV-1 virus variants from all donors showed the lowest number of AA differences with the subtype B consensus in Gag and the highest number of differences in Env, confirming their respective highly conserved and variable nature.
The median number of total IPI-O variants from all of the isolates was 5, and most of those variants belong to class I, with a median number of 4. However there were obvious exceptions, with Guatemalan isolates #46 and #68 containing 17 and 14 IPI-O variants, respectively.
We developed a straightforward and simple protocol for extraction of basic proteins from the adipocytes, and using controlled proteolytic digestions of these proteins and LC/MS/MS we revealed recovery of histone variants from all subfamilies, namely H1, H2A, H2B, H3 and H4, in amounts sufficient to make up to 100 nanoLC/MS/MS analyses.
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The human genome variant background was generated as control group by subtracting four types of disease-associated variant from all SNPs that appear in the dbSNP database.
In HIV-1 variants isolated from all recipients 9 22 months after the transmission event, a limited number of additional reversions had occurred, again both in- and outside predicted donor-HLA-restricted epitopes (Figure 1, right panels, white stacks).
Moreover, each of these rare variants differed from all other sequences by a single non-synonymous (amino acid altering) substitution at an otherwise invariant site.
Variants (Single nucleotide polymorphisms (SNPs) and Insertion/Deletions (In/Dels)) in coding as well as non-coding regions were then called from each alignment file using GATK [ 36], and the overlap of variants identified from all three alignment files was used for further analysis.
To build Venn Diagrams, the following filtering criteria were used: for somatic SNVs we required a minimum of 20X coverage depth with ≥5 variant reads from all tumor and normal samples, for indels we used coverage depth cutoff of 20 and variant reads of five for tumor samples.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com