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Lipid vesicle cosedimentation showed that both truncated variants exhibited altered binding with phosphatidylinositol (PI).
The last four variants exhibited altered dynamic interactions among members of the nucleotide salvage pathways and the non-oxidative branch of the pentose phosphate pathway.
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Engineering of secreted protease variants exhibiting altered substrate specificity is a challenging task because effective screening methods for the desired property are not available yet.
For a network-centric approach, we may find that carriers of high-risk variants exhibit altered brain activity in a specific cortical network or an abnormal connection between two such networks.
However, after 60 min, cell surface expression of the truncated hIPΔ312 and hIPΔ307 variants differed significantly from the hIP with evidence of altered trafficking or recycling, i.e., they exhibited altered time-dependent movement to and from the cell surface followed by further internalization, and after 4 h incubation both hIPΔ312 and hIPΔ307 were located at the cell surface.
Five of the six single amino acid substitutions of CBS selected for physical analysis exhibited altered sensitivity to the B6 cofactor in vivo (the sixth, functionally benign K267M variant, was included in this set as a control), but displayed differing phenotypes with respect to heme-level sensitivity.
Again, the ZmN transgenic plants exhibited altered responses different from the wild type plants.
Once plated, colonies exhibited altered morphology (Fig. 1).
However, DKO mice exhibited altered pain sensitivity to an acute thermal stimulus.
Neither KO nor Het mice exhibited altered operant responding for food as a reinforcer.
Therefore, we aimed to identify genes that exhibited altered expression in breast, prostate, or both cancers.
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