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As expected for large proteins (>60 kDa), all hu3S193 variants exhibited a hepatic clearance.
We recently demonstrated that T phase variants exhibited a higher growth rate and greater epithelial adherence and destruction than did O phase variants during interactions with human middle ear (ME) epithelial cells.
Although each VSFP2.1 variant showed a significant component of donor emission in the acceptor channel, this problem was most pronounced in the case of the CFP/YFP pair since the two other variants exhibited a larger separation of donor and acceptor emission spectra.
All of the Gln → X variants exhibited a significant decrease in kcat.
In general, the more severe, chronic G6PD variants exhibited a 'disrupted' pooling structure of the network.
Accordingly, compound heterozygote patients carrying these variants exhibited a severe Alpers syndrome (Table 1).
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Surprisingly, the P10P8 and P14P12 mutants were not significantly different in the early 'fast' phase from the ATC232S control, and the P6, P4 and P6P4 variants exhibited an increased rate despite their repression of polymerization (k1,cd in Table 3).
All activated pro-TGase variants exhibited an optimal pH activity profile (pH 5.0 7.0) similar to that of the recombinant wild-type TGase (Fig. 6a) but more acidic than that of TGase naturally secreted by S. hygroscopicus (pH 6.0-7.0) [ 18].
Other variants exhibit a transient increase in fluorescence during the 10 ms phase followed by a decrease during the rate-limiting phase.
Interestingly, the most common PTGER3 haplotype ATAAA (rs2206344, rs3765894, SNP_A-4228934, rs2744918, rs2268062) was associated with the risk for hypertension (P = 1.6×10−5, OR = 1.50; hypertensives 57%; normotensives 46%), whereas two other prevalent variants exhibit a protective effect (Figure 6B).
Four selected IgG variants exhibiting a range of affinities for Fas receptor were then characterised for their apoptotic activity.
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