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This finding indicates that these variants displayed only a fraction of their maximal whole cell fluorescence under the conditions in which library screening was performed (80 μM BV).
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The wild-type (Pro187Ser) allele of NQO1 was identified by a 191 bp band while the homozygous variant (Ser/Ser) and the heterozygous variant (Pro/Ser) displayed only a 151 bp band and two bands (191 bp and 151 bp), respectively.
Interestingly, the pathogenic TPI variant Ile170Val displayed only about 30% of the wild-type catalytic activity; hence, this activity is sufficient to suppress the growth defect of Δtpi1 yeast on glucose medium.
Cells expressing the variants mined out of the AY library displayed only a fraction (16 69%) of the fluorescence signal obtained with cells expressing full-length IFP.
Of 127 individuals with TLR4 variants two patients displayed the Thr399Ile allele only, and three displayed only the Asp299Gly allele.
An IGF1R-fl variant with a cysteine substitution immediately following the Fn3 domain and further away from the TM (Q895C) displayed only a modest increase in basal phosphorylation compared to wild-type, perhaps owing to steric interference from Fn3 domains.
That was displayed only fleetingly.
The compounds displayed only modest JAK1 inhibition.
Neurology and psychiatry displayed only a slight interest from the 1890s in the broad delineation of "hysteria"—the nosological classification within which Osler conceptually located AN and other variants of psychical mind-body issues.
However, several of the clones matching ESTs and annotated transcripts displayed only partial overlap with the known sequence, suggesting that the subtracted clone may still represent an unidentified splice variant of the known transcript.
No cells displayed only selamectin-induced currents.
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CEO of Professional Science Editing for Scientists @ prosciediting.com