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In that study, transcriptional analysis of htrA levels in opacity variants demonstrated that transparent strains produce more htrA transcript than the opaque variant during late-exponential phase.
Dose-response curves for salicin and 4-NP-β-mannoside with wild-type TAS2R16, and N96T and N96A variants demonstrated that N96T resulted in a greater than 5-fold decrease in EC50 for both compounds relative to wild-type TAS2R16.
A close comparison of the two VAR2CSA DBL5ε recombinant variants demonstrated that, despite a homology of 80% in their amino acid sequences, both variants presented some distinguishable characteristics.
Nevertheless, the proteinase K digestion of all P66 variants demonstrated that the protein was exposed on the surface of the bacteria.
Nevertheless, a systematic analysis of mutant variants demonstrated that they perform opposite functions in the control of seed germination [ 23- 25].
In vivo and in silico characterization of the variants demonstrated that we obtained psd modules with increased degradation rate and higher dark/light ratio.
Similar(52)
However, experiments using GR variants demonstrate that two proteins binding the same response element can undergo dynamic exchange with short protein DNA residency times and, in fact, do not compete51.
Circular dichroism and nuclear magnetic resonance spectra of all engineered variants demonstrate that they maintain their native conformations despite substantial sequence modification.
Quantification of abundance of histone variants demonstrates that H2AB is the most abundant variant in the mouse hippocampus.
This series of variants demonstrates that it is possible to tune psd module stability to desired values with appropriate combinations of destabilizing and stabilizing mutations.
Quantitative fluorescence spectroscopy of yeast cells expressing the sensor variants demonstrates that they accurately report transporter activity as 30 50% FI decrease of green FI (De Michele et al., 2013).
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