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This produced two lists of variants (based on the SAMtools pileup files), one for each genotype.
That is, it is a haplotype-based caller that identifies variants based on the sequence of the reads themselves instead of the alignment, the latter of which is how GATK's UnifiedGenotyper operates.
freebayes [ 30] also uses Bayesian methods to detect variants, but is haplotyped-based, in the sense that it calls variants based on the literal sequences of reads aligned to a particular target, not their precise alignment (https://github.com/ekg/freebayes).
Suspected germline variants based on the allele frequency of normal samples were filtered out.
Mutagenesis, expression and screening for the identification of genetically engineered ArMs variants based on the biotin-Sav technology.
The non-redundant set of PacBio subreads were aligned to the assembly, and corrections were applied by calculating the maximum likely variant allele for the phased variants based on the read depth.
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An acoustic power computation finally validates the implementation of a fast variant, based on the perturbation technique, for vibroacoustic applications.
Recombinant eADF4(C16) represents an engineered spider silk variant based on the sequence of the core domain of the natural dragline silk protein ADF4 of Araneus diadematus.
The Old Believers, who split off from the main body of the church, continued to use the archaic Great Russian variant, whereas Patriarch Nikon's new variant, based on the southwestern tradition, was adopted by the official church and is used in it to this day.
The individual scores from this analysis are weighted based on the perceived relative importance of each of the criteria against the original specification and selects a turbine variant based on the total weighted score.
Pakistan's Navy took delivery of six Mk.45 Sea Kings, a variant based on the Royal Navy's Sea King HAS.1 from 1975.
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