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Strikingly, we found that both Gli2 variants are localized to the cilia (Fig. 4C and Table 2; n = 32/32 for Gli2SD1-3 and 31/34 for Gli2SD1-4).
If ΔdinB strains expressing DinB variants lacking the β-clamp binding motif are as sensitive to either MMS or NFZ as ΔdinB, then it could be inferred that the observed enhanced sensitivity is mediated through interactions with the β-clamp, and is consistent with the idea that these DinB variants are localized at the replication fork.
Four of these variants are localized in homopolymeric regions of three or four repeats.
All of the Robo2 variants are localized to axons when expressed pan-neurally with elav-GAL4.
In addition, structural studies of microbial communities (e.g. biofilms; Wilmes et al., 2008b) may show whether particular variants are localized within distinct microniches.
To further address whether or not our Robo2 deletion variants are trafficked normally in vivo, we have added a supplemental figure demonstrating that all of the receptor variants are localized to CNS axons and are present at comparable levels.
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In subjects S1, S2 and S3 a well-supported subclade of R5-using viral variants was localized in PBMCs and distinct from quasispecies in contemporaneous tissues where X4 and R5 variants commingled (Fig. 1a, 1b, and 1c).
Based on the UCSC in silico PCR search (https://genome.ucsc.edu/cgi-bin/hgPcr), no common variants were localized to the two primer sequences.
Most MC1R variants were localized in six receptor domains: transmembrane 1, 2, 3, and 7, intracellular 2, and C terminal domains.
After introducing the expression plasmids into the Halobacterium sp. NRC-1 Δ gvpC strain, GvpC protein and variants were localized to the GVNPs by Western blotting analysis and their effects on increasing the size and shape of nanoparticles established by electron microscopy.
Accordingly, the abcD-CPY* variant was localized exclusively to the ER (Figure 4, panels n1 to n3).
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