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Mutation screening of 93 TOF cases revealed no VEGF coding sequence variants and no changes at splice consensus sequences.
We found read support for all six variants and no evidence of other alleles at these positions.
Consequently, there is little selective pressure for more efficient sulfate reduction genes resulting in more variants and no dominant variants.
As shown in Figure 8, IRF-7C is associated with other IRF-7 splicing variants, and no obvious splicing-specific expression was observed in either IFN-α or Sendai virus treated cells.
The corresponding OR for three or more variants and no detectable adducts was 0.93 (95% CI, 0.56 1.56).
Similarly, group D had extensive radiation of ITS2 variants and no relationships among strains were resolved (Additional file 4).
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Poly(A) signal hexamers can be classified into three types: AATAAA, 1 nt variants of AATAAA (1 nt-variants), and no poly(A) signal (noPAS) (Additional file 1: Figure S5).
However, reoxidation of the His174Ala variant protein resulted in an absorption spectrum with characteristics of the Cys166Ala variant, and no 6-thio FAD was observed.
Steady-state enzyme kinetics showed that, as expected, the variants were severely impaired with only ca. 0.2% activity (as judged by kcat/ Km) for the Y137F variant and no detectable activity for the Y137A variant (Table 1).
Similarly, 14 cases and 13 controls carried an innocuous rare splice junction variant and no other potentially more severe rare variant, resulting in an OR of 0.62 (P = 0.25) (Table 3).
Furthermore, the same tests were performed in subgroups stratified for the presence of the HLA-DRB1 shared epitope, PTPN22-1858T and TRAF1/C5 rs10818488-A vandano, associationsiations were detected (data not shown).
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