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To further enhance the anti-HIV efficiency of these ribozymes by increasing their level of transcription, we designed several tRNALys3 promoter variants and compared their expression levels from the internal tRNALys3 promoters and also from an exogenous human U6 snRNA promoter.
We called sequence variants and compared the results to the existing data for the HapMap samples as well as to data in publicly available databases and validated the findings by Sanger sequencing.
We transformed GFP targeting sgRNA, sg(C1), into E. coli K12 coexpressing the aforementioned Cas9 variants and compared resulting colonies to a control nontargeting sgRNA, sg.
Objectives: We hypothesized an adaptation to high-arsenic living conditions via a possible positive selection for protective AS3MT variants and compared AS3MT haplotype frequencies among different indigenous groups.
Furthermore, we examined biological processes enriched for genetic variants and compared these biological processes between this Quarter Horse mare and the reference Thoroughbred mare.
Except Arg587Arg SNP, the frequencies of other 17 polymorphisms were inferred lower than 1%, and we pooled all the rare variants and compared the frequencies (Table 2).
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Here, we investigated the genetic polymorphisms in MTDH by direct sequencing in a cohort of breast cancer cases and controls, with the intention of discovering of novel variants and comparing the distribution of SNP genotype frequencies of these 2 affected vs. non-affected cohorts to determine whether a particular SNP may influence susceptibility to breast cancer development.
We could potentially pool these variants and compare the aggregate frequency of these variants to increase power.
The weighted-sum method also combines rare variants and compares the burden of mutation between cases and controls to determine genes or pathways of interest [ 29].
To run this analysis, one simply aggregates carriers of rare variants and compares their phenotype mean or disease prevalence (for quantitative or dichotomous traits, respectively) with non-carriers.
Therefore, the objective of this section is to explain hair colour in the Corpas family using different SNP variants and comparing the genotypes of the family with other European populations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com