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We also assessed the folding properties of one resulting variant when expressed in the endoplasmic reticulum of HEK293 cells and explored possibilities of using Sapphire as a donor protein in FRET-based genetically encoded indicators.
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Taken together these findings indicate that α2AAR and its tagged variants when expressed in THP-1 cells induce similar responses as endogenous chemokine receptors.
We next investigated the functional consequences of variants when expressed in the SH2B1α isoform.
Next, we examined the localization of TDP-43 variants when expressed in motor neurons using the D42 Gal4 driver (Fig. 3A F).
Localization of the Cx43 variants when expressed in REKs revealed that almost all cells were in fact expressing the Cx43 variants.
The team examined the phenotypic effects of several ALS-linked TDP-43 variants when expressed in neurons or glia, compared with wild-type TDP-43.
Eighteen sites were combined to produce one CEL7A variant, which when expressed in T. reesei had a Tm increase of 14.8°C (Tm 76.0°C) as determined by DSC.
The morphology of the low metastatic cell variant MDA-MB-435Br1 MDA-MB-435Br1 MDA-MB-435Br1e is indicative of a less invasive cell.
Consistent with this, we were not able to demonstrate any dominant interfering affect of 1100delC variant when ectopically expressed in 293T cells.
Notably, this is the first report in which a number of these DinB variants have been studied when expressed from the chromosome.
Because T. reesei is a preferred host for enzyme production, we developed it as the host for screening to ensure that expression and performance of the selected variants would not be lost when expressed in the production host.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com