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In addition, human induced pluripotent stem cells (hiPSCs) derived from individuals with autism containing a CEP290 variant were used.
The Salmonella strains harbouring SGI1 or variants of it used in conjugation experiments are described in Table 1. S. enterica strains 959SA97 (serovar Agona harbouring SGI1), 47SA97 (serovar Agona harbouring the SGI1-C variant), 7205.00 (serovar Albany harbouring the SGI1-F variant) were used as donor strains [15].
Antibodies specific for the HV2 loop of each Opa variant were used as positive controls (0.87 1.2 µg/mL) in all IFA assays; polyclonal rabbit antisera against heat-killed FA1090 (1∶1,000) was used as a positive control for Opa-negative variants; negative controls were antibodies against heterologous HV2 loops and wells that were not incubated with primary antibodies.
A set of hard-filter thresholds, which includes the Phred-scaled quality scores (QUAL, −10log10 P variant / no variant)), were used for evaluating InDels.
Samples from patients with the known genotype CY2D6*1/*1 (EM or wild-type variant) were used to create the standard curve for the real-time PCR analysis.
To test the role played by MEK5/Erk5 activation in progression of breast carcinoma cells to a resistant phenotype, MCF-7 cells (N variant) were used to stably express a constitutive active MEK5 construct.
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L159V variant was used to convert HPAH to l-homophenylalanine (l-HPA) in the hydantoinase process.
Further, the proposed variant is used for optimizing the clustering process.
The enzymatic activity of CMTr1126 550 was confirmed in vitro (Supplementary Fig. S1); consequently, this protein variant was used in the crystallization trials.
This P2Ox variant was used for the bioconversion of l-sorbose under O2-saturation in a molar scale.
The AdaboostM1 variant is used for this research.
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