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The turbulent quantities such as the mean temperature, turbulent heat flux, and temperature variant were obtained by DNS.
When single hyphal isolates were obtained from the tuft resulting from the pairing between homokaryon A and heterokaryon AB, only the heterokaryon and a variant were obtained.
Additional "torsionally unrestrained" and "torsionally restrained" sub-variants of each building variant were obtained by changing the mass distribution, while total mass sum remained unchanged.
Crystals of the ceN[S123E] variant were obtained at 4°C using the sitting drop vapor diffusion method.
Good diffracting crystals of the FDC_Es E72A variant were obtained after approximately two months in the presence of ammonium sulfate (0.2 M), Bis-Tris (0.1 M, pH 5.5) and PEG 3350 (25% w/v).
The miRNA identifier and corresponding pre-mir substructure for each variant were obtained by matching variant location with pre-mir coordinates retrieved from miRBase [ 32]. miRNA variant analysis was performed by custom R [ 33] scripts.
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The sequence of this truncated variant was obtained by mass spectrometry analysis.
During the preparation of homokaryons through protoplast regeneration and tuft formation, a defective homokaryon was detected and a new variant was obtained.
Moreover, a new colony type variant was obtained from the tuft resulting from the pairings between the homokaryon and the heterokaryotic parent.
Human KIAA0457 (DISC1 long variant) was obtained from Kazusa (RIKEN).
A synthetic gene encoding the MsDef1-γ4 substitution variant was obtained from GenScript Corporation (Piscataway, NJ) and subsequently cloned into pET28a and expressed in the Rosetta (DE) strain of E. coli as described elsewhere [44].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com