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Expression plasmids coding for the ATG16L1*300A variant were generated from their *300T counterparts by site-directed mutagenesis.
The 3′UTR of PXN mRNA and a mutant variant were generated by q-PCR and cloned to the XbaI site of a pGL3-basic vector (Promega) and termed PXN-wt-3′UTR and PXN-mt-3′UTR, respectively.
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The mouse-adapted variant was generated in the serial passage experiments described Bell et al [21].
When a new H3N2 variant is generated within the seeding region it competes for susceptibles with existing variants.
The truncated 206 ABCB6 core variant was generated by PCR.
A glycoengineered variant was generated using the methods described above.
However, how the PEDV variant was generated and has evolved is not clear.
At the same time, the CCR-negative variant was generated by knocking out the ptsG gene.
The frequencies of each variant are generated dynamically through bar charts.
Once the user selects which residues to back-mutate and submits them to the system, a new variant is generated and the process can be repeated.
The ACDha variant was generated by the cysteine to dehydroalanine reaction as described above and then exchanged into gel filtration buffer without BME.
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