Sentence examples for variant were constructed from inspiring English sources

For the quantitative real-time PCR assay, two standard curves specific for each splice variant were constructed.

Pro247-Ser variant was constructed based on homology modelling and rational design.

An additional D8S1179 reverse primer specific for the variant was constructed resulting in the recovery of the null allele.

For this purpose, a Gag variant was constructed encoding a 105 kDa protein fusion containing Gag at its N-terminus (to ensure in vivo VLP assembly and GFP encapsulation), followed by an 11 amino acid T7 epitope tag (for immunological detection) and a factor Xa cleavage site to release mature GFP from Gag (Figure 6A).

To maximize the destabilizing interactions, the R141E/Y288A/A306F variant was constructed.

The H107AH108A variant was constructed as previously reported.

For this reason, the R103K variant was constructed to investigate the possibility that a lost charge-based stabilization was responsible for the increased KM in the R103Q variant.

Each variant was constructed using the program tleap by stripping off the side-chains and substituting them with the new amino acid, natural or modified.

In place of native truE, a truE variant was constructed in which patellin 3 was present in the first core peptide cassette and a randomized NNK library was present in the second cassette.

The corresponding Atg8ΔR variant was constructed by site-directed mutagenesis using the follow primers: Forward, ACA TTT GGC TAG CAG TCT TTT ATA TG, Reverse, AGA CTG CTA GCC AAA TGT ATT TTC TC.

A FLAG-tagged HOT1 homeobox deletion variant was constructed by PCR amplification of the pcDNA3.1-FLAG vector using primers with site-specific overhangs (Table III; Metabion) following the QuickChange II Site-directed mutagenesis kit protocol (Stratagene; Zheng et al, 2004).