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To address this issue, we conducted a synchronized assay where Vero cells were exposed to HSV (MOI of 0.1) at 4°C for 2 h and increasing concentrations of C5A or the non-amphipathic control C5A variant were added just prior to shifting the temperature to 37°C (Figure 3).
To test for secondary association signals at each locus, genotypes at the most significant variant were added to the logistic regression model as a covariate, and all other variants at the locus were tested.
Under the first β setting (β1 = β4 = 3, β5 = β6 = β7 = β8 = − 3), since the cumulative effect sizes β s on each single rare variant were added to zero, the haplotype-based tests outperformed the rare variants based tests.
Lastly, amino acid substitutions in a given GltA variant were added sequentially with energy minimization to an RMS gradient of 10-3 kcal/mol/Å using the Amber12 force field with Extended Hückel Theory and R-field solvation electrostatics after each mutation.
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On the basis of these reports, atypical type A thymoma variant was added to the type A thymoma family as a small subset of aggressive tumors [5, 6].
An atypical type A thymoma variant was added to the WHO classification of type A thymoma family in 2015 [5] based on several reports about type A thymoma with oncological aggressive behavior and tumor relapses [2 4].
A simulated variant was added to two genes, GLI3 and CAV2.
The shorter variant was defined as standard (100%, component A) and the longer variant was added as equal or minor contribution (component B).
Seqnature scans through the variant call format (VCF) files, and at each position with a known SNP or indel, if the sample haplotype matches the founder strains with the variant, the variant is added to the individualized genome.
This is exemplified in Figure 3 where the dashed gray line represents the risk profile for the most common genotypes modeled from the 12 asthma susceptibility genes (group 1 variants) and the solid blue line shows the risk profile when the NAT1 variant is added.
Four more variants were added over the years: Spider Solitaire uses two decks and ten rows of cards; Pyramid has the cards set up in a pyramid, obviously, and you remove pairs of cards at a time; and TriPeaks has three pyramids which you have to clear.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com