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In the V19I variant, we observed have half-lives of 17 min at both illumination conditions.
For the d5f variant, we observed a progressive increase in the levels of constitutively spliced transcripts from day 7 (2.3%), peaking at d22 (63.9%).
Glycine residues are known to destabilize β-sheets [ 32] and in one out of three MD simulations of the I1067G G-box variant we observed large fluctuations in the inter-strand twist angle and increased loss of hydrogen bonds near the substitution, which resulted in the complete breakage of the β-sheet.
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During initial analysis of LRR variants, we observed that it was possible to distinguish two groups of variants on the basis of their effect on structure or function.
By running 100 simulated datasets each with 10 or 20 variants, we observed that the algorithm found the correct minimal p-values in all cases.
After excluding non-deleterious variants, we observed 32,213 unique deleterious variants occurring 105,323 times.
Initially, we assessed whether the variants we observed were located within known transcription factor binding sites (TFBSs), namely NF-κB, ATF-2 and SMAD-3.
If the variants we observed do generally enhance Spy's chaperone activity, then why has evolution not already come up with this solution?
In the 5.6 M variants, we observed 23.3% heterozygous compared to 72.7% homozygous calls, which was not expected from the Illumina® MaizeSNP50 genotyping data.
Remarkably, none of the variants we observed correspond to the variants described in an earlier study, in which a PR8 strain (originally adapted for growth on embryonated chicken eggs) was adapted for growth on MDCK cells [ 78].
When restricted to unambiguous events transmitted by individuals likely to carry only PRDM9 A or B variants (see below regarding PRDM9 variants), we observed that 51% (26/51) of NCO events overlap a DSB hotspot.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com