Utilizing a naturally occurring splice variant, we demonstrated that the C-terminal domain of Mnk1 restricts its interaction with eIF4G, preventing eIF4E phosphorylation in the absence of MAPK signaling.
Furthermore, using different Rab5 truncation variants, we demonstrated that the N-terminus (residues 1−15) and C-terminus (residues 185−215) of Rab5 are dispensable for interaction with APPL1.
In those individuals with no genetic variants, we demonstrated up to an approximate 2-fold variation in levels in keeping with previous studies (Fig. 2) (37).
Although multiple PML splice variants exist, we demonstrate that the ratio of a subset of cytoplasmic PML isoforms lacking exons 5 & 6 is enriched in cells exposed to herpes simplex virus-1 (HSV-1).
Using both Pulse Field Gel Electrophoresis (PFGE) and the disappearance of the phosphorylated form of the histone variant H2AX, we demonstrated that the ability to repair DSBs is increased during adipocyte differentiation using the murine pre-adipocyte cell line, 3T3F442A.
Herein, using the murine bladder tumour cell line MB49 and its more aggressive variant MB49-I, we demonstrate that the adaptive immune system efficiently limits progression of MB49, whereas MB49-I has lost tumour antigens and is insensitive to adaptive immune responses.
The use of our tool on a publicly available whole-exome sequencing cancer dataset demonstrated its uniqueness in the identification of germline and somatic variants: RAREVATOR was able to identify more than 1500 germline and 22 somatic RRA variants that we demonstrated to participate to significantly mutated pathways.
Comparing the degree of rescue obtained with either human SHANK2-WT or the three variants, we can demonstrate that only the R462X variant fails to rescue the excessive branching to a normal level, an effect that fades out 75 µm away from the cell body (Fig. 3B and C).
In this paper, firstly we report the discovery of a new splice variant in rodents, and secondly we demonstrate that both PIP5KIγ93 (now PIP5KIγ_v3, as suggested in Ref. [7] – see below) and this new variant are present in humans.
