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Hence, apart from the monophonic and the predominant variant, we also investigate the approaches w. r. t. usual polyphonic onset detection (all onsets).
On each variant, we also used hydrazide chemistry for attaching fluorophores at oxidized carbohydrates.
For the last model variant we also fitted a version which includes non-assortative mixing between species (see equation (8)).
To evaluate the effect of the genotype containing the SNP variant, we also conducted analyses under dominant model.
For each human variant, we also inferred the ancestral and derived states by comparing it to the orthologous non-human primate sequence (Fig. 1d).
In those individuals without a rare genetic variant, we also assessed FI levels in the context of a common CFI variant previously associated with AMD (rs10033900) (12, 40).
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For follow-up confirmation of identified novel variants we also applied capillary sequencing.
Besides spectral variants, we also included dipole orientation mutants and constructs with duplicate (tandem) acceptors in our study.
Besides considering the most significant variants, we also tried to relax the significance threshold, allowing more markers to be fitted.
To avoid unfairly inflating the variance in probes because of uncertainty in the predicted breakpoints of known copy number variants, we also excluded probes within 25 kb of a predicted breakpoint.
Aside from these rare variants, we also observed the common amino-acid substitutions I97V, R462Q and D541E.
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