Even though we detected many different variants between our strains and the reference sequence, subtraction of strain specific background variants was sufficient to remove almost all non causative variants.
From these data it will also be possible to estimate whether a liability threshold of common variants is sufficient to cause schizophrenia, and whether this can be applied to individual risk prediction.
One theoretical model suggests that mutation occurring in neutral gene variants is sufficient to block their fixation in large populations, leading to a large flux of transient novel sequences (Berg & Kurland, 2002).
With regard to the third criterion, some investigators have argued that in the context of a high-risk family, the difference in risk between carriers and noncarriers of clearly pathogenic CHEK2 sequence variants is sufficient to justify a difference in cancer surveillance strategies [ 68- 70].
Mammary epithelial cell-targeted overexpression of genetically engineered STAT5 variants is sufficient for initiation, promotion, and progression along a cancer pathway, resulting in mostly well-differentiated adeno-carcinomas, sometimes exhibiting a papillary architecture, and a low percentage of undifferentiated carcinomas [ 60, 66, 67].
Interestingly, we found that dynein interacted with the same region of SNX4 as CHC, but the clathrin box variant was not sufficient for dynein binding.
We checked whether the amount of off-target mtDNA reads was sufficient for mtDNA variant detection.
The fraction of bases mapping to the targeted exonic regions was fairly stable for all samples (approximately 60%) and the overall coverage depth per sample was sufficient for reliable variant calling.
Average depth of coverage in all candidate regions of shared homozygosity was sufficient for comprehensive variant detection (29- to 114-fold, Supplementary Material, Table S1) of all exons and for all known annotated genes within all candidate regions regardless of their size.
Our power analyses show that a number of N = 90 per genetic variant will be sufficient to obtain a type I error rate of 0.007 and a power of 0.8.
