R462Q is located in the kinase-like domain, and the R462Q variant is capable of binding 2-5A, with a reduced ability to dimerize, a configuration necessary for the enzymatic activity [3].
Whether a protein which interacts with a specific SR in one variant is capable of interacting with the same SR in additional variants is yet to be examined.
The H131 but not the R131 variant is capable of interacting with IgG2 and has been previously shown to bind and mediate phagocytosis of IgG2-coated particles [ 18].
Unlike the wild type allele, the threonine residue of the mutated variant is capable of being phosphorylated, and there is evidence that while phosphorylation sites are important for activation of proteins in signal transduction pathways phosphorylation sites can act at the same time as signals for degradation by ubiquitin ligases.
At the highest concentration used, the combination of selective variants was capable of killing almost 95% of cells upon 24 h incubation.
These RGD-containing AcAP5 variants were capable of interacting with platelet receptor αIIbβ3 as shown in computational analysis.
Based on the fact that multiple rare MEFV variants were capable of causing FMF if present on both chromosomes, we decided to test the transmission of rare variants from parents to offspring collectively.
Interestingly, both N-WASP variants were capable of driving actin tail formation, although reduced efficiency of N-WASPΔA was reflected e.g. by increased expression levels required for inducing prominent actin tails (not shown).
In order to determine which variants are capable of inducing T cell responses, the DQ2-Glia-α1, DQ2-Glia-α2, DQ2-Glia-α3, and DQ8-Glia-α1 variants were synthesized as 15- or 16-mer peptides and tested for their capacity to bind to HLA-DQ2 and induce in vitro proliferation of HLA DQ2- or DQ8-restricted T cell clones.
A preliminary bioinformatic analysis showed that both DNA variants are capable of forming G-quadruplexes, however, with varied stability.
