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The presence of several of these variant transcripts in human brain was supported by our RT-PCR experiments, which showed that they are expressed at low levels, similar to FAK transcripts containing exon 13.
Consistent changes in the same direction are observed in the homozygous Sod n108 line with only the levels of the SOD3 variant transcripts in females being significantly reduced (n=5, P<0.05; Figures 7C and 7D).
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We analyzed the expression of the variant transcript in Northern blots of fetal and adult mouse (Fig. 4).
This led us to reassess ZFY transcripts in the human testis where we have identified a testis-specific splice-variant, structurally homologous to the major Zfy1 variant transcript in mice.
In contrast to the Northern analysis, where brain did not have detectable expression of the Il4i1_2 variant, RNA in situ hybridization revealed expression of the variant transcript in the adult mouse brain (Fig. 6).
One of variant transcripts found in mammary gland samples was missing exon 12; this variant was also reported in human BRCA2 [ 22].
PDE4A super-short form splice variant transcripts were detectable in EST libraries of S. scrofa, B. taurus, R. norvegicus, M. musculus, C. jacchus, and H. sapiens.
ADAM-12L (membrane-bound long variant) transcripts were overexpressed in tumours when compared to controls, while no expression of short form of ADAM-12 (ADAM-12S) (secreted short variant) was detected in the lung tissues examined.
Splice signals within HERVs can also result in variant transcripts of cellular genes.
Reference and variant transcripts with accession number in the array are shown.
By way of contrast, Rnf14 variant 3 transfection did not lead to a detectable change on the expression of mitochondrially-encoded mitochondrial genes, despite an overabundance of the variant 3 transcript in the variant 3 transfected cells.
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