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Both the WT hBChE standard curve and hBChE variant samples were resolved on the same gel by sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred to nitrocellulose.
In total, 17 NV GII variant samples were confirmed using NKII-F/R/R2, SRII-1/2/3, and GII-F1/R1/F2.
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d, Since experiments to determine the translation activity of mtIF2(H678A) were performed at a later time point than for other variants, samples were analysed on separate immunoblots.
Since these are absolute (non-normalized) counts, however, the method may not work if the variant sample is obtained with a different method, e.g. by rRNA depletion of total RNA whereas most normal samples would come from polyA+ libraries.
MPC with time-variant sampling is a reliable tool for the implementation of TGC in patients in the medical ICU.
For the 5557G>A variant, 50 samples were genotyped by two independent methods (minisequencing and Amplifluor™ fluorescent genotyping).
For the variation in intron 17, for which no restriction site could be modified by the variation, common and variant homozygous samples were discriminated by dHPLC of a mixture of a known common homozygous sample with the unknown sample.
Among imputed variants, 45,802 variants from 5,349 samples were compared to the corresponding variants obtained from an exome chip constructed using identical samples.
Genetic variants for both samples were available for variants on the Cardio-Metabochip (Illumina).
14 PTC (10 classical forms, 3 follicular forms and 1 diffuse sclerosis variant) and matched samples were used for quantitative RT-PCR validation.
Variants in tumor samples were first identified by SAMtools [ 30] for each patient, and only variants supported by at least three reads with base quality ≥20 were retained.
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