Sentence examples for variant reading of from inspiring English sources

This statement reads like that of a Protestant heretic who defies the Pope, only to insist on his own variant reading of Christian doctrine.

Additional file 5: Consistency of percentage variant reads of SNPs.

The bi-allelic SNPs and in-dels identified were further filtered to minimum unique variant read of ≥4.

To build Venn Diagrams, the following filtering criteria were used: for somatic SNVs we required a minimum of 20X coverage depth with ≥5 variant reads from all tumor and normal samples, for indels we used coverage depth cutoff of 20 and variant reads of five for tumor samples.

We excluded variants with minor allele frequency (MAF) >1 % in control databases (Exome Sequencing Project [ESP] and 1000 Genomes) or with MAF >2 % in our cohort and variants of low quality, i.e., with depth of coverage <20 and/or with the ratio of variant reads to total reads <0.2.

During variant calling we required a minimum coverage depth of 20 and minimum number of variants reads of 5 to call SNVs or indels.

For each potential point mutation, we extracted those with coverage reads more than 10 and a ratio of variant read to wild-type read more than 0.6.

Only sites containing over a minimum number of variant reads (default of three) are considered in the subsequent analysis (initial variant calling).

These included four of 412 concordant substitutions (3243A<G, 9854T<G, 3645T<C and 15222A<G) with <span class="lh">variant read frequencies of 19% to 64% and two of seven discordant substitutions (16093T>C and 7501T>C) with frequencies of 14% and 18% (Table 2).

SNVs were called using Varscan v2.2.2 (ref. 18) with a minimum total coverage of 5 reads and a minimum variant read depth of 3. The mitochondrial variants were annotated using Annovar v.21-Feb-2013 (ref. 19) and its mtDNA-specific database files (hg19_MT_GRCh37).

We note that copy number gains or losses in tumor genomes may alter the expected variant read proportions of germline variants, so this two-step approach will not always exclude germline variants.