In a variant procedure, a defeated animal is left in chronic contact with the olfactory cues of the aggressive intruder, such that exposure to a psychological stressor is chronic, although the actual agonistic experience is intermittent.
Cesium and strontium ions removal from aqueous solution onto nano-sized zeolite was investigated using uni-variant and multi-variant procedures.
Multi-variant procedure was used to optimize the removal process and deduce equation to predict the removal efficiency.
Variant discovery procedures were then applied using whole-genome recalibrated alignments across both CxA and Lovell sample simultaneously using GATK HaplotypeCaller tool applying standard hard filtering parameters [ 45].
Others reported failure rates of 20 to 60%, even if variant surgical procedures, such as Mohs micrographic surgery, improve results with recurrence rates lowered down to 16% [ 12].
Although alternative variant calling procedures can result in different number of variants, the consistency of our datasets composition (Supplementary Table S1), in terms of total SNVs, PDVs and PIGs, makes us confident about the average quality of nsSNVs used in our analysis.
Using this technique we genotyped a fragment of Mhc class I exon 3 of several hundred great tits simultaneously; and applied a stepwise variant validation procedure to separate true alleles (the procedure was based on the methods defined by Zagalska-Neubauer et al. [ 23] and Galan et al. [ 28]).
We applied a stepwise variant validation procedure to detect true alleles (Table 2); the method is broadly based on the procedures defined by Zagalska-Neubauer et al. [ 23] and Galan et al. [ 28].
After applying the stringent variant filtering procedure, we obtained a total of 39,898,568 autosomal variants (Ti/Tv ratio = 2.11 2.14).
The predictive ability of the model was estimated by cross-validation and by external predictions for new HIV variants; both procedures showed very high correlation between the predicted and actual susceptibility values (Q2 = 0.89 and Q2ext = 0.86).
More advanced haplotype-based methods incorporate the correlation between adjacent variants within the variant detection procedure.
