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This study is the first report of a flower color variant of wild soybean G. soja discovered in nature.
We propose that p53-246 degrees may represent an allosteric variant of wild type p53 compatible with positive growth control.
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We have studied the kinetics of the nucleotide-induced conformational changes in a single-tryptophan variant of SR1 Y485W-SR11) and compared the resulting data with those we reported previously for the double-ring, single-tryptophan variant of wild-type GroEL (Y485W-GroEL).
pEGFP-N1 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.
pEGFP carries a red-shifted variant of wild-type GFP (GFP mut1) with a C-terminal His-tag, which has been optimized for brighter fluorescence.
In contrast, the expression of a mRNA splice variant of wild-type uPAR (uPAR-wt) lacking exons 4 and 5 (uPAR-del4/5) has been demonstrated to be a highly sensitive, independent prognostic marker in breast cancer patients [ 14- 16].
The vector pEGFP-N1 encodes a red-shifted variant of wild-type GFP that was modified for brighter fluorescence [ 56] and which was used as a reporter to identify transfected cells by flow cytometry.
The finding of CC homozygote (72P) carriers having poorer survival is consistent with the R72 variant of wild-type p53 being a more potent inducer of apoptosis than the wild-type 72P variant.
For instance, eGFP is a widely used mutational variant of wild-type GFP owing to its superior brightness, photostability and relatively small sensitivity to pH (Cormack et al., 1996).
pEGFP-N3 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in somatic cells of livestock and poultry, which had been demonstrated in our early studies [ 26, 27].
The open reading frame (ORF) of a human codon-optimized variant of wild-type Zoanthus sp. green fluorescence protein (ZsGreen) was subcloned from the pZsGreen-N1 vector (Takara Bio, Shiga, Japan) into the pQCXIN retroviral vector (Takara Bio), and the resulting construct was designated pQCXIN-ZsGreen.
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