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Rhythmically, he leans on urgent repetition and the plunging momentum of his band, working with rhythms traceable to one or another variant of fusion.
However, as there may be additional variant of fusion mutation, FISH may be better than PCR method in terms of sensitivity.
Furthermore we constructed a series of plasmids for each splice variant of fusion genes studied here for optimizing all the probes.
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These variants in the form of fusion proteins, carrying 46 amino acid residue-long ABD sequence linked to a helical 305 amino acid TolA spacer protein with an installed C-terminal AviTag consensus, were further characterized.
(C ) Activation of the Btk-Abl fusion construct (1 μM) and that of a variant of this fusion protein in which the Btk PH-TH module is mutated (Y42R/F44R).
Figure 4 shows a broader phylogenetic sampling of TrpAa sequences than shown in Figure 3. Figure 4 illustrates the distinct cohesion of TrpAa domains of TrpAaTrpAb fusions, as well as tight clustering of another variant of TrpAaTrpAb fusion denoted TrpAaTrpAb_phz.
Chmielecki et al. found 7 variants of this fusion transcript in 29/53 SFT (55%) [ 37].
In the present study, the baits for sequencing were designed to capture all breakpoint variants of ALK fusion gene.
These results are fully consistent with the results of our previous kinetic experiments performed in vitro with asymmetrically-mutated variants of the fusion complexes.
Additional baits were designed from intronic region between exons 19 and 20 of ALK gene to capture all breakpoint variants of ALK fusion gene.
This makes simple assays using, for example, PCR difficult if one wants to identify or exclude all variants of the fusion gene.
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