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The three-dimensional structure (3D) of a hybrid mammalian recombinant (rePON1) variant obtained by directed evolution has been determined, providing the first structural information of this protein.
The A1-5A variant, obtained by replacing the IRS sequence with alanines, still inhibited translation activity (A1-5A, Figure 1b), indicating that the IRS-penetratin sequence had no role in translation inhibition.
In the present work, the consequences of P-gp transfers in MCF-7 were studied by using, as a P-gp donor, a cell line variant obtained by selection for resistance to doxorubicine (Doxo) and therefore called MCF-7/Doxo.
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A set of 73,728 ICP variants obtained by combination/permutation of 26 methods are evaluated.
The basis for the altered DNA specificities of two Cre recombinase variants, obtained by mutation and selection, was revealed by their cocrystal structures.
Stability data of the AtPLA2 variants obtained by fluorescence or CD measurements of the reversible unfolding by guanidine hydrochloride and urea showed that all enzyme variants are less stable than the enzymes from animal sources although a similar tertiary core structure can be assumed based on molecular modeling.
Additional topics related to PDL include results concerning comparative power of expression, decidability, complexity, and completeness of a number of interesting variants obtained by extending or restricting PDL in various ways.
Conversion rate constants of psd module variants obtained by parameter estimation.
From the variants obtained by exome sequencing, we selected nonsynonymous variants in a prespecified list of 56 SF genes or 1423 autosomal recessive disorder genes.
To establish the database, all relevant data from Human Gene Mutation Database (www.hgmd.org) and sequence variants obtained by literature search for "ARC," "VPS33B," and "VIPAR" were collated.
Instead, an additional band at around 800 bp appeared, which did not correspond to any of the splice variants obtained by sequencing (Fig. 1).
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