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The ID number of each novel variant corresponds to the variant number displayed in Table 1.
We did the simulations in an environment with variant number of obstacles.
Thus, besides using 'class' number as the estimate of splice variant number, we have also used a weighted method such that a class was counted as 0.5 splice variant if it followed the above two situations.
The analysis using the new AS variant number revealed that although the mean AS variants for each AP group was less than those of our previous analysis, genes with more APs always had a higher number of mean AS variants (Supplementary Figure S1).
Applying these filters, the initial variant number was reduced by a factor of 10.
Variant number 4 (hereafter designated ZEB1sh-ET) displayed the greatest ZEB1 knockdown and commensurate CDH1 protein reexpression.
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We used 'class' numbers extracted from AltSplic to represent the AS variant numbers in the above analysis.
Using the AS variant numbers estimated from the Ecgene dataset also supported the positive relationship between the number of APs and the number of AS variants.
Gene-level information includes gene IDs, gene-level P values, significance level AS, gene region lengths, total variant numbers, significant variant numbers and statistics for diverse effects.
Sequence variant numbering was based on the transcript ENST00000392069 for PAX3 and ENST00000314557 for MITF.
PorA allele and variant numbers were assigned and sequences were deposited in a database (http://hercules.medawar.ox.ac.uk/momp).ac.uk/momp
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