Sentence examples for variant library of from inspiring English sources

To synthesize and identify the custom-made LacI variants for sensing these four new inducers, the team designed a novel workflow incorporating a combinatorial synthesis strategy that relies on computational protein design and the Wyss Institute's custom DNA synthesis resources to build a variant library of potential new biosensor designs comprising hundreds of thousands of mutated LacI proteins.

As an example, a limited library of enhanced green fluorescent protein (EGFP) were designed to encode two variant codons (YAT at Y66 and TWT at Y145), the product of which would encode a limited variant library of green and blue variants of EGFP (Fig. 13).

(A) Residues for generating the single-site variant libraries of the aldehyde-binding site are shown using the KGSADH structure derived from homology modeling.

The opportunities afforded by these biocatalysts are increased by the availability of greater diversity provided by the genomic resource and the variant libraries of well-known P450s produced by rational and random engineering techniques.

In the present study, we generated a glyco-variant library of HRP C1A exchanging all the eight Asn serving as glycosylation sites by the structurally similar amino acids Asp, Gln or Ser.

With respect to the overall purification efficiency for the glyco-variant library of HRP C1A using a two-step flow-through approach, we observed vastly different results (Table III).

An added advantage to this method is the inclusion of ultrahigh-throughput FACS-based screening which aids in the rapid screening of the variant library [ 51].

Importantly, any mixed base codon can be added to incorporate into the designed sequence, hence automating the design of the variant library.

Thus, protein improvement is likely to involve the creation of increasingly 'smart' variant libraries over larger parts of the protein.

In contrast to random methods (error-prone PCR or DNA shuffling), site-directed mutagenesis enables the rational design of variant libraries and provides control over the nature and frequency of the encoded mutations.

As a result, there is significant interest in the development of novel methodologies that can address these issues to produce accurate variant libraries, with larger numbers of simultaneous mutations in an economical workflow.