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The top-ranked variant is retained and its UPCs added to those of the original motif.
The general picture that can be drawn from these data strongly suggests that the greater inhibitory effect on IR autophosphorylation exerted by the Q121 variant is retained at most downstream post receptor steps, thus resulting in a more profound inhibition of the entire insulin signaling pathway in both cell types.
However, it does not localize to the plasma membrane, but this naturally occurring variant is retained in the endoplasmic reticulum, a phenotype previously known from many podocin mutants.
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The tally for each variant was retained with the sequence name for further analyses.
For tair9, the first identified splice variant was retained in the absence of canonical splice information.
If isoforms for a gene existed in the predicted peptides of the Theridion species, only the longest variant was retained.
In all procedures, indel calls were filtered according to the Phred quality score; only the calls with the PhredScore > 10 for an alternative variant were retained.
We cloned the coding sequence from a human kidney cDNA library and showed that the expressed short variant was retained in the endoplasmic reticulum while still associating with detergent-resistant membrane fractions in sucrose gradient density centrifugation.
Upon synthesis, most familial mutant PrP variants are retained and aggregated in the ER and Golgi, where they may exert their pathological effects (review in [12]).
After filtering for genes with available annotation, 5647 genes and 2956 variants were retained for analysis.
So all the EMS variants were retained that showed a high GQ (30).
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