Research on an Olympic long jumper from Spain showed that he had no copies of the R variant, indicating that athletic success is probably affected by a combination of genes as well as factors like environment, training, nutrition and luck.
Substitution of Trp164 by His also resulted in an inactive variant, indicating that an indole side-chain is required for activity.
Functional analysis of a recombinant FhCatL5 L69W variant indicates that the residue at position 69 is important for the S2 subsite architecture and can influence substrate specificity.
In agreement with previous results, this abridged variant indicates that the helical domain is not necessary to preserve the general topology of IFABP's β-barrel and that the helix-turn-helix motif is a fundamental element of the portal region involved in ligand binding and protein membrane interactions.
The variant did not form tuft when paired with parental heterokaryon AB or homokaryon A. Its protoplast regenerants gave rise to heterokaryon AB, homokaryon A and the variant, indicating that it is a new kind of heterokaryon.
When protoplasts were obtained from the variant isolates, the regenerants gave rise to heterokaryotic parent, homokaryon A or the variant, indicating that they belong to a new kind of heterokaryon.
In a reciprocal fashion, fibril formation is impaired when the amyloidogenic segment is fused to a stabilized CspB variant, indicating that stabilization of an adjacent folded domain inhibits fibril formation.
Interestingly, we were also able to complement the delay in fruiting-body formation in the Δcas1/2 strain by overexpressing either cas1, cas3, or a cytoplasmically-located cas2 variant, indicating that the function of CAs is redundant for fruiting-body formation in S. macrospora.
Recombination events and point mutations may be reinforced by positive selection for the new variant, indicating that the new oligopeptide variant may have biological significance [ 25].
