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The K35BPA variant displayed the best performance, with nearly 50% of all hIgG1 and hIgG4 heavy chains cross-linked, exceeding that of the previously reported Q32BPA variants.

With 80% of mIgG3 heavy chains labeled after only 1 h of UV exposure, the L17BPA variant displayed the best cross-linking performance ever reported of any Protein Z or Protein G based conjugation technique.

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Figure 3a c show the number of variants displaying the resistance type "+" towards DMSO; out of 371 beneficial substitutions 126 were to polar (34%), 117 to charged (32%), 88 to aliphatic (24%), and 40 to aromatic amino acids (11%).

Nevertheless, these variants display the fastest aggregation kinetics, which suggests that the polyQ tract also affects the mode of JD aggregation [14].

Among these histone variants, the macroH2A variants display the most unusual protein structure: an N-terminal H2A domain is fused to a large C-terminal non-histone domain.

However, F1 progeny of stable true-breeding phenotypic revertants and bal variants display the morphology of heterozygous BAL/ bal plants, arguing against the possibility that any suppressor mutations are dominant mutations tightly linked to RPP5 locus (Data Not Shown).

Finally, taking into account the low genetic diversity existing within unigenes and CDS predicted from the BAC clone HM061166.1, the finding of alignment variants displaying the insertion or deletion of gene sequence traits in the unigenes provides a first indication for the presence of splice variants in sequences encoded by genes surrounding the HAPPY locus.

mECFPΔ-Epac(CD, ΔDEP -cp173Venus-Venus ΔDEP -cp173Venus-Venus ΔDEP -cp173Venus-Venusbest FRET span and signal-to-noise ratio, but due to the complex decay kinetics [7], [8] and moderate quantum yield of mECFP it is less suitable for quantitativariant.

In addition, the A134F variant displayed an approximate fourfold increase in the rate of butanal consumption (kapp value of 0.003±0.0005 min−1) and approximately sixfold increase in pentanal consumption (kapp value of 0.023±0.001 min−1) compared to wild-type cADO.

The R72A variant displayed nearly wild-type binding affinity for the substrate.

In the latter study, 7 individuals with the P147Q variant displayed a clefting phenotype among a total of 16 carriers with this variant.

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